FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10604049
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG467240.4406241427213322No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG195880.18472189255255234No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA134370.1267157479185545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA13050.0115.566431
GCAGGTA35850.071.209789
CGAAAGC29950.062.6344073
GAGCAAA132550.061.6258051
CAATTCG7300.055.321089
AGCAAAA160450.050.997242
GCAAAAG159800.050.884283
GCAGGGT88800.047.345769
TCTACAC25350.037.2846263
GAAAGCA51550.037.0909584
CTACACT26400.035.256294
AGCAGGG161200.033.865418
GTTACGT1501.1777538E-633.6746181
GTCATAC25250.032.8649061
GGTAGTT17450.032.6483547
AAAGCAG304200.032.3234566
GTAGTTT18500.031.5746678
ACAAGGG61950.030.9796733
CAAAAGC278500.029.9231724
AAGGGTG52300.029.3835415
Produced by FastQC (version 0.11.5)