FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_PLASMID_CONTROL_P2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_PLASMID_CONTROL_P2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012459
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA31320.30934585993111824No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT29020.28662889065137453No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG27550.27210978419866877No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG26380.26055376069549485No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG26250.259269758084031No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG24800.24494819049462746No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC23920.2362564805093342No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT23760.23467616960291723No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT23430.2314167783584323No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA19380.19141515853975322No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG19090.1885508450218725No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT17460.1724514276627498No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT17240.17027850016642648No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG17160.16948834471321803No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT16570.16366094824580552No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAACCTTTAGGAA16530.16326587051920127No Hit
TCATATAGGAGACCGATTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC16220.16020401813801843No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA16050.15852493779995042No Hit
GATATACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGA16000.15803109064169513No Hit
GAATAAGAGAGGCTATCTAATAAGAGCTTTGACATTGAACACGATGACCA15970.15773478234674196No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAATTTGCAGGTCCT15870.15674708803023135No Hit
GTATAAGTTTGGTCCCCCATCTGATACCAATAGTCCTGCCCTTGATTGGG15810.156154471440325No Hit
CTCTTATTCACTCTTTGTTTTTTCTTCCCTATTGTTCTTTGTGTGACCAT15130.14943815008805295No Hit
ATGTAATCATCGCCAAAAACATTCGAGGGTTTTGATTTTCATTCCACTTA14410.1423267510091767No Hit
TCCTTGAAGAATCCCACCCAGGTATCTTTGAGAACTCATGCCTTGAAACA13940.13768458772157688No Hit
AAATAAAACAGGGACATTTGAATTCACAAGCTTTTTTTATCGATATGGAT13790.13620304624681098No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT13560.13393134931883663No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT13490.1332399632972792No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC13410.1324498078440707No Hit
GGCTAATAGATTTCCTCAAGGATGTGATGGAATCAATGGATAAAGAGGAA13300.13136334409590908No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT13180.13017811091609635No Hit
TCCCTGAAGTCTGCTTAAAGTGGGAGCTAATGGATGAGAATTATCAGGGA13170.1300793414844453No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG12650.12494333103859019No Hit
GTTTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAATTT12570.12415317558538173No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT12520.12365932842712643No Hit
CTATTAGCCTTCCTGATTCATTAGCTGTTAGTCCATTCGATCTAAAAACT11930.11783193195971392No Hit
TTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCT11890.11743685423310968No Hit
ATATGTAATCATCGCCAAAAACATTCGAGGGTTTTGATTTTCATTCCACT11760.11615285162164592No Hit
GCATTAGCCAACACCATAGAAGTTTTTAGATCGAATGGACTAACAGCTAA11720.11575777389504169No Hit
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC11580.11437500185192684No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC11350.11210330492395248No Hit
AATAAGAGCTTTGACATTGAACACGATGACCAAAGATGCAGAGAGAGGTA11340.11200453549230142No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG11230.11091807174413977No Hit
CCATAGAAGTTTTTAGATCGAATGGACTAACAGCTAATGAATCAGGAAGG11220.1108193023124887No Hit
GTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATA11180.11042422458588447No Hit
AACTTATACAATATCCGGAACCTTCACATCCCTGAAGTCTGCTTAAAGTG11180.11042422458588447No Hit
CATAGTGAATGCACCAAATCATGAGGGAATACAAGCAGGAGTGGATAGAT11100.10963406913267598No Hit
ATCCAGGAGTGTGTAGTTGCAACGGCATCATATTCCATACTTTTGGCTGG11050.10914022197442069No Hit
GATTACATATATCACAAAAAATCAACCTGAGTGGTTCAGAAACATCCTGA11000.10864637481616539No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT10920.10785621936295692No Hit
TTATTGGTGCGATGCTCAGGATGTTTCTGAACCACTCAGGTTGATTTTTT10910.10775744993130586No Hit
GTCCTATATAAATAAAACAGGGACATTTGAATTCACAAGCTTTTTTTATC10800.1066709861831442No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC10730.10597960016158678No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG10650.10518944470837832No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACACCATG10650.10518944470837832No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG10590.10459682811847196No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA10550.10420175039186773No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG10400.10272020891710183No Hit
CCATATATGGGCTATGAACTAATGACCCCGTAATTGATTACTATTAATAA10300.10173251460059124No Hit
TTCACTATGAGGGCAAAATCGTCGGAGGATTGGAGCCCATCCCACCAGTA10240.10113989801068488No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTGA10190.10064605085242959No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT10140.10015220369417428No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG100.0070955856144.96783
GGAGTGA100.007099778144.93916145
AGGCCCC301.602806E-596.62611145
CGACGCC300.001938974872.469574145
CCCATCG604.95053E-448.3225983
AATAATT1704.0017767E-1142.6270567
ATAATTA1755.2750693E-1141.409148
GCTGCAC907.4923104E-540.268833
TAATTAA1807.2759576E-1140.258899
TTATACT11700.039.029794
CCTTATA11800.038.6990282
CTGCACA951.0302462E-438.1494184
TACTGAT10250.037.4702227
TATACTG22350.037.294125
GCCAATC1001.3932807E-436.241951
ACTGATG10400.036.2329988
TACTGGA12700.035.94777
ACTGGAG12900.035.3903738
GTATATA10050.035.340411
CTGATGA10700.035.217129
Produced by FastQC (version 0.11.5)