FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_564-2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_564-2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011407
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG16200.085.896622
AGCGAAA16550.084.0800861
CGAAAGC17750.078.395793
GAGCGAA6000.073.6832051
TATACGA1650.048.2964367
TACGAAC1801.8189894E-1244.2717329
GAGCAAA37700.043.8312031
TTACGCA850.002747230234.1060142
CAAAAGC47250.033.2849464
GCAAAAG48650.032.4760783
AGCAAAA55350.029.1996152
GAAAGCA61050.024.3364984
CGTACAA1801.5263556E-424.154226
AAAGCAG95600.023.804055
CGTAGAC2353.721357E-521.588383
AAAAGCA80650.020.2188875
ATATACG4553.4615368E-919.1110326
AAGCAGG113700.018.1634656
GTATACG3252.2112448E-517.8400691
GTATTAT23900.017.5881441
Produced by FastQC (version 0.11.5)