FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_563-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_563-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427852
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG25600.1792902905903413No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC21700.15197653538321898No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA21490.1505057947182201No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG21280.1490350540532212No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG19850.13902001047727636No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA17260.12088087560895666No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT15550.10890484447967996No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA15440.10813445651229958No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA14320.10029050629897217No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4450.0115.632461
CGGTATT300.001941378672.448497
AGCGAAA9250.061.11311
GTTACGT1450.059.9783741
CGAAAGC9650.058.5799183
GCGAAAG10000.056.5296172
GTTCGAC400.006060050354.35542
ACGGTAT400.0060617354.3515936
GAGCAAA40750.050.8650931
GATATAC3150.050.616673
TACGTGT1750.049.6963653
TAATCGT901.330478E-648.2989929
CGAAGCG1850.047.0100755
TTACGTG1900.045.772972
CCGACTA5050.043.053784
CACCGAC5050.043.053782
CAATTCG1201.9852996E-742.2616169
AAGCAGG59700.042.231287
AGACGAC1859.640644E-1139.1750643
GTTATGT8250.038.6527251
Produced by FastQC (version 0.11.5)