FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_562-3_v2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_562-3_v2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682870
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG24410.14504982559555998No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG23870.14184102158812031No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC23700.14083084254874115No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA18790.11165449499961376No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT18670.11094142744240494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8500.070.767791
GTTACGT3650.061.552451
TTACGTG3650.059.5668872
TACGTGT3550.059.2015723
AGCGAAA11600.058.7281571
GCGAAAG12050.058.3392942
TATAACG502.014931E-457.974995
GAGCAAA45950.056.6220321
CGAAAGC12700.054.7811433
GCGACTA1602.0008883E-1145.2943084
ACGTGTT4900.042.8909384
CAAAAGC60900.042.3639374
TTAGACG1053.8362577E-641.4119384
GTAGCAT19100.038.702881
GCAAAAG67050.038.262043
AGACGAC2750.036.894273
AAGCAGG83700.036.874967
AAAGCAG86450.036.6292846
TAGACGA1209.596066E-636.2343675
AGCAAAA71050.036.0070152
Produced by FastQC (version 0.11.5)