FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_561-3_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_561-3_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588581
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG157750.9930245923877976No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA114060.7179992710475576No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT48300.3040449306645365No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG41010.2581549193903238No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA40050.2521117903336374No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC40000.25179704402860165No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC38240.24071797409134316No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC37380.23530433764472822No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC36210.22793927410689163No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT35290.2221479420942338No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC35110.2210148553961051No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT34890.21962997165394776No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT33980.2139015889022971No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT33720.21226490811611115No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG32460.20433330122921023No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT29700.1869593051912367No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA29560.1860780155371366No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC26230.1651159116217555No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG26130.164486419011684No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT25740.16203139783240514No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC24920.1568695584298188No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA24660.15523287764363292No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT24390.15353324759643985No Hit
GAATCTCCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC24260.15271490720334688No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA24210.15240016089831115No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT23520.14805666188881775No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT22400.1410063446560169No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA21660.13634809934148778No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC21620.13609630229745917No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT21490.13527796190436622No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG20980.13206754959300154No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG20770.1307456151118514No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA19990.12583557275329366No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC19660.1237582471400577No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG19640.1236323486180434No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT19640.1236323486180434No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA19610.12344350083502195No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT18990.11954064665257863No Hit
AGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGA18840.11859640773747138No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC18750.11802986438840701No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG18690.11765216882236411No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT18630.1172744732563212No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC18380.11570074173114243No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG18190.11450470577200658No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC18180.11444175651099944No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG18150.114252908727978No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG18080.11381226390092794No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC17450.10984646045747745No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA17300.10890222154237021No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT17260.1086504244983416No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA17090.10758028706122004No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC17080.1075173378002129No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT16940.10663604814611279No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA16780.10562885996999838No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA16730.10531411366496264No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG16560.10424397622784108No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA16540.10411807770582679No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC16020.10084471613345494No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC16010.1007817668724478No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT16000.10071881761144065No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA15890.100026375740362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCG100.0071006627144.93571
TGTCGAC100.0071006627144.93572
GTAACCC100.0071006627144.93571
TCGAGCA100.007101331144.931124
GAGCGAA8200.0139.633161
ACCAGGT203.873794E-4108.698355
GCAGGTA24150.089.679749
TACCAGG259.405503E-486.958694
GAGCAAA76600.083.53671
AGTAGAA9300.080.260091
TTATAGG2200.079.055831
GCAGGGT46950.077.601969
CACCTAC3600.076.491433
CCACCTA3750.073.434092
AGCAAAA87450.072.9236152
AGCGAAA16700.072.901791
CCTACTA3850.069.642235
GCAAAAG91950.069.51023
TCCACCT4050.067.994521
GCGAAAG18450.066.379762
Produced by FastQC (version 0.11.5)