FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_559-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_559-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394350
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA11550.087.848881
GCGAAAG12350.081.571412
CGAAAGC13400.075.179633
GAGCGAA5550.053.5403251
GAAAGCA34550.029.7872494
GAGCAAA27400.026.1863381
TATTCGT1751.262357E-424.8388799
CAAAAGC27350.024.3792674
GTATTAG6000.024.158441
AAAGCAG67000.022.2834575
GTCGCAC1650.00261249421.9535549
TAGAGCG2003.1193474E-421.734029
GCAAAAG32350.020.611223
GTTACGT2155.0691323E-420.225671
ATAATAC16750.019.4709823
AGCAAAA33900.019.4550272
TATATAC14950.018.9066073
AAGCAGG82300.017.9627886
GCAGGCG4908.691131E-917.7420588
GTGCGGA2050.009166880517.6699359
Produced by FastQC (version 0.11.5)