FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_540-2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_540-2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784232
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA55600.062.313251
GAGCGAA13000.061.8880731
GCGAAAG16800.058.2440832
CGAAAGC17000.057.9852183
AGCGAAA17800.054.5647471
CAAAAGC70200.049.043774
GCAAAAG70750.048.3551643
TCGTCGA450.00965007748.321021
AGCAAAA75950.045.0444762
AAAGCAG103400.041.2119566
GTTACGT2252.0008883E-1135.435411
AAGCAGG114650.035.3220527
AAAAGCA98800.035.0640225
TCGCACG850.002747120834.1060835
GACGACT4050.028.6346764
AGACGAC3900.027.8775083
GCGACTA1050.00771963827.612014
CGAAGCG1606.8664966E-527.166869
CGACTCC3800.026.6999766
TTACGTG3451.09139364E-1025.2109642
Produced by FastQC (version 0.11.5)