FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_531-7_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_531-7_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119457
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG41850.3738419608792477No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA28870.2578928891417893No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC18960.16936782743776668No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA18600.166151982612999No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG18190.16248949267368018No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA18060.1613282153758474No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG15400.13756669528173032No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC15180.13560145677770563No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT14400.12863379299070887No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA13510.1206835099516998No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC13490.12050485190587938No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12980.11594907173745841No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT12430.11103597547739662No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG11780.10522958898823269No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT11430.10210307318637517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5050.0116.242421
TTACGTG1900.080.100772
GTTACGT1950.078.04691
TACGTGT1950.078.04693
GTCACGT1350.075.156271
GAGCAAA34400.071.84011
GCGATTA1350.069.76304145
AGCGAAA9050.064.063752
TATTCGC350.003572053562.1189581
GCAGGTA10850.061.4290549
CGAAAGC9450.061.352064
GCGAAAG10050.059.8525963
GGGTTAC1251.8189894E-1257.9776951
AAGCAGG57000.053.1272287
TCACGTC1650.052.7069972
CGTTCTG553.2267103E-452.7022865
GCAGGCG553.230983E-452.688169
ACTCCGA1258.913048E-1152.1799281
GTAGAAA9950.050.985411
GCGACTA901.3272311E-648.3147474
Produced by FastQC (version 0.11.5)