FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_529-7_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_529-7_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241718
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12860.10356618813611464No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5200.086.4143751
GAGCAAA41100.056.7821351
AGCGAAA7850.055.3961031
CGAAAGC8150.054.2462733
GCGAAAG8900.051.3036423
CAAAAGC49300.046.6025854
GTTACGT2350.043.1775321
GCAAAAG54550.041.9845963
AAGCAGG66100.041.534417
AAAGCAG67600.040.414746
AGCAAAA57950.039.5213052
ATCTCGC1752.4374458E-937.2736661
TAGTCGG1951.72804E-1037.1480148
TACGTGT2750.036.8971633
TTACGTG2900.034.988692
AACGGGT2302.7284841E-1134.662713
GGTTATA3600.034.2250481
CGAAGCG1707.883864E-834.0887689
AAAAGCA71350.032.30085
GCAGGGG32800.029.8146849
Produced by FastQC (version 0.11.5)