FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_526-7_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_526-7_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281862
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG98660.769661632843473No Hit
GTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAA74850.5839162093891542No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA68290.5327406538301315No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC37820.2950395596405853No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT30800.24027547427102136No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA29020.22638942413457921No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT27480.21437565042102819No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC26080.20345403795416356No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT25920.20220585367223617No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG25640.20002153117886323No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC24710.19276646004016035No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG22990.17934847900944095No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT22880.17849035231561589No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT22740.17739819106892943No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT22230.1734196036702859No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC21760.1697530623421242No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC20990.16374617548534864No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC19780.15430678185327282No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT19710.15376070122992958No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG18710.14595954946788342No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT18500.14432130759785375No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT18490.14424329608023329No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG18330.1429951117983059No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT17620.13745629404725315No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG17580.13714424797677127No Hit
ATATTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGG17440.13605208673008484No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG17330.13519396003625975No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA16800.1310593496023753No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA16440.1282509349680387No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT16370.12770485434469545No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC16270.12692473916849084No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG15980.12466240515749745No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC15620.12185399052316084No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT15610.12177597900554038No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA15060.117485345536415No Hit
GTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTACATTGTGGAAACAC14920.11639318428972854No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA14880.1160811382192467No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT14560.11358476965539194No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC14280.111400447162019No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC14130.11023027439771207No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA14110.11007425136247116No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA13350.10414537602331608No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG13270.10352128388235239No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG13250.10336526084711148No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC13060.1018830420123227No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG12820.10001076558943162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4350.0144.96551
CCGCATC100.007096139144.96554
CCACGAA100.007101935144.92592145
GCAGGTA13950.097.652379
TATCATA301.6015229E-596.643673
AGTAGAA7150.090.223291
TTATAGG3700.088.154711
TCGCTCC259.396231E-486.979315
AGCGAAA9750.078.058351
GCAGGGT26000.077.1979149
GCGAAAG10650.071.461872
CGAAAGC10800.070.4693453
GCAAAAG59550.065.4843
GACAGTA1350.064.409017
GAGCAAA62700.062.425341
AAGCAGG73250.062.1228187
CAAAAGC62600.061.946124
GTAGAAA11200.057.5979042
AAAGCAG78700.057.3738676
AGCAAAA69250.056.9395182
Produced by FastQC (version 0.11.5)