FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_526-2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_526-2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377169
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA42750.076.115721
GAGCGAA10300.066.138561
GCAAAAG55950.057.6400073
AGCAAAA55950.057.510482
CAAAAGC56800.057.032624
GCGAAAG13100.056.4277842
CGAAAGC13750.054.287353
AGCGAAA13650.053.623221
AAAGCAG84000.046.928476
AAAAGCA72750.044.5269475
AAGCAGG100950.036.4529157
CTGTACG1352.155071E-532.1952939
GTTACGT1902.0848529E-730.5140951
GGTAACG1400.001001989125.8824922
TCGATTA4400.024.695251145
GCAGGGG40150.024.356969
TAACGAA2104.3269433E-420.7059944
AGCAGGA34200.020.1220558
GTATTAT20300.019.6349951
TTACGTG3001.109846E-519.3255962
Produced by FastQC (version 0.11.5)