FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_420-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_420-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939285
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC14700.1565020201536275No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA14630.15575677243861022No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG13180.14031949834182383No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA12420.132228237435922No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12330.13127006180232836No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC10090.1074221349217756No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG9900.10539931969530014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2850.0104.2461241
AGTCGGC1650.074.659645
CGAAAGC4050.067.990694
AGCGAAA4400.064.229252
GCGAAAG5000.059.4202843
TATACTG3600.058.373595
GATATAC3250.055.741363
GTTACGT1700.055.4134671
AGTCCGC1850.054.822845145
TACGTGT1800.052.3349383
TCGGCTG2450.050.2675937
CTCGCAA752.558012E-548.3091773
GAGCAAA24000.048.3091771
GGCCGTA450.0096580148.3091742
TTACGTG2000.047.1014442
GTAATGC1255.2186806E-946.376811
GTCGGCT2750.044.7910166
GCGATTA1401.4239049E-841.396843145
CAGTCGG3000.041.06284
CGGCTGG3050.040.378898
Produced by FastQC (version 0.11.5)