FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_199-5_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_199-5_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916756
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG25870.13496762237864393No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG25380.1324112197901037No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA23100.12051612203118184No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC22920.11957703536600382No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20450.10669067946050516No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC20050.10460382020455394No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG19980.10423861983476247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7100.097.998141
GAGCAAA65800.061.7933771
GTTACGT2700.061.740341
TTACGTG2750.060.617792
AGCGAAA11750.059.832731
CGAAAGC11900.059.075453
TACGTGT2850.058.4877933
GCGAAAG13300.054.4917343
AGACGAC3500.047.625783
CAAAAGC86600.046.4469684
CGAAGCG2700.045.612749
AAGCAGG103200.045.487947
AGCAAAA90250.044.4102132
GCAAAAG92600.043.202653
AAAGCAG111200.042.5500456
AGTCCGC3450.041.996384145
GGTTATA4400.041.180561
GGTCCGC750.001489394938.63667145
GGTCGTT951.03395716E-438.1282967
GTATACG1157.1611703E-637.81451
Produced by FastQC (version 0.11.5)