FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_199-2_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_199-2_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590975
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG39650.24921824667263784No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA27290.17153003661276892No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA22120.1390342400100567No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC19540.12281776897814234No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC16540.1039614073131256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4100.0123.750551
ACGGTAT300.001938778272.473356
AGCGAAA8750.068.754811
CGAAAGC9000.067.6503143
GTAGTCG350.003570308462.127841
GCGAAAG10250.059.400272
GAGCAAA49500.049.7859421
ACTCCGA1501.0913936E-1148.3216481
AAGCAGG63950.045.884927
GATATAC2550.045.47923
GTTACGT2100.044.8701021
TACGTGT2100.044.8701023
GCAGGTA11650.044.7777339
TTACGTG2150.043.826612
TAGTCGG2200.042.813058
GGTCGTT1256.0936145E-1040.57367
CAAGGTA4650.040.5112729
GCAAAAG64250.038.243673
AGTCGGC1901.2914825E-1038.1486745
CGAAGCG1157.1572285E-637.8169445
Produced by FastQC (version 0.11.5)