FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_196-3_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_196-3_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543439
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG19750.12796100137420396No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC18680.12102843066684203No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG17810.11539166756833279No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA17220.11156903512221734No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA16910.10956053332849564No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16030.10385897984954379No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3750.096.619141
TACGTGT2800.090.5804443
GTTACGT3050.085.53171
TTACGTG3100.081.81462
CGAAAGC6800.062.8734863
CGTAATT350.003576578862.10023145
AGCGAAA7250.059.9705051
GCGAAAG8550.050.852182
GAGCAAA44700.048.1474571
GCGACTA1557.4396667E-1042.076084
GATATAC4900.039.929343
CTCGCAA1105.2839787E-639.5260123
AACGGGT3900.039.019273
GGTCGTT1303.7210157E-739.010427
AAGCAGG63100.038.92217
GTAATCG750.00148722638.6476553
CGAAGCG2850.038.1304869
ACTCCGA2151.2732926E-1137.0747831
AGTCGGC2750.036.8909455
ACGTGTT6900.036.7572824
Produced by FastQC (version 0.11.5)