FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_179-7_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_179-7_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568999
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG39910.2543660002332698No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA27240.17361387738296838No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC23210.14792871123563495No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA22000.14021678790107578No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT20750.13224992495215102No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC20750.13224992495215102No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC19850.1265137836289252No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT19320.1231358337385811No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT17020.10847680591255954No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT16930.10790319178023695No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT16260.10363295323961327No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC15850.10101982219236597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4500.098.251641
AGCGAAA8850.078.6231461
GCGAAAG9900.069.549982
CGAAAGC9950.068.472063
GAGCAAA51200.048.9811061
GTAGAAA13250.048.6851581
TACGTGT1700.046.897793
CGAAGCG1402.728484E-1046.590295
GCAGGTA10900.045.8632779
CAAAAGC55750.045.2421234
GCAAAAG55550.044.7526443
GTTACGT1903.6379788E-1241.962521
AAGCAGG70100.041.961457
AGCAAAA61950.040.1292842
AAAGCAG76200.038.8023876
TAGAAAC17200.037.9247442
CTTAGAC4400.037.8864363
GTCATAC7500.036.723561
CATACTC7700.036.709843
CTACACT8950.036.4416544
Produced by FastQC (version 0.11.5)