FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l02_n01_TR_122-7_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l02_n01_TR_122-7_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981902
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA13500.1374882625761023No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG13390.13636798784400073No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12980.13219241838798576No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG12620.1285260647192897No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC12220.12445233842073852No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10960.1116201005803023No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT10480.10673162902204089No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA10450.10642609954964956No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG9930.10113025536153301No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA100.0070992713144.94241
GAGCGAA2400.0126.824591
GTTACGT1350.069.787081
TTACGTG1450.064.9741742
CGAAAGC5200.062.7154543
AGCGAAA5450.059.8385961
TACGTGT1600.058.8828473
GATATAC2200.056.0004653
GCGAAAG6200.053.768952
CGAAGGG1353.6379788E-1253.679635
GAGCAAA27950.052.635611
AAGCAGG35700.049.3140647
GGTTATA2250.048.3141331
CGAAGCG1850.047.0059475
CAATTCG1453.8744474E-1044.968389
GCGAAGC1950.044.597664
GTCACGT1652.910383E-1143.9219361
ACTCCGA1053.8345097E-641.412111
AAGGTCG907.502138E-540.2597245
AGTCCGC2005.456968E-1239.84698145
Produced by FastQC (version 0.11.5)