FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11135825
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG178310.1601228467580983No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA142610.12806415330700688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA26150.090.9868851
GAGCAAA167250.063.7136271
GCAGGTA60550.058.1537489
GCAAAAG190600.055.6259773
AGCAAAA193100.055.019472
CGAAAGC47500.054.3512573
GCAGGGT100050.052.284839
CAAAAGC253000.042.310724
AGCAGGG168400.041.1330538
AAGCAGG334250.038.9560857
AAAGCAG345000.038.0566986
AAAAGCA319850.033.4214975
GGTAGTT19450.033.156537
TTTAAAC21100.030.588683
CAATTCG12800.030.5660559
GTAGTTT21350.029.8648228
GTCATAC21050.028.6024131
GAAAGCA92850.028.119344
GGTCGTT11100.027.4173187
ACAAGGG68550.027.3996833
Produced by FastQC (version 0.11.5)