FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_PLASMID_CONTROL_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_PLASMID_CONTROL_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411242
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA64980.2694876748165468No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG58860.244106564169005No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT57820.23979343425504368No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG55950.23203809489051702No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG55390.22971564032146086No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG51260.21258753787467208No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC49840.20669845664599407No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT46590.19321992566486482No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT45600.18911415776599777No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA44750.18558900350939475No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA41480.1720275277222278No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG39200.16257181983392793No Hit
GTATAAGTTTGGTCCCCCATCTGATACCAATAGTCCTGCCCTTGATTGGG36270.1504204057494022No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT36230.15025451613732674No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT35900.14888592683770438No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAATTTGCAGGTCCT35740.14822236838940264No Hit
GAATAAGAGAGGCTATCTAATAAGAGCTTTGACATTGAACACGATGACCA35730.1481808959863838No Hit
CTCTTATTCACTCTTTGTTTTTTCTTCCCTATTGTTCTTTGTGTGACCAT35480.1471440859109123No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG35250.14619022064147855No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT35210.1460243310294031No Hit
ATGTAATCATCGCCAAAAACATTCGAGGGTTTTGATTTTCATTCCACTTA34920.1448216313418562No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAACCTTTAGGAA34760.14415807289355442No Hit
TCCCTGAAGTCTGCTTAAAGTGGGAGCTAATGGATGAGAATTATCAGGGA32800.13602948190185807No Hit
GATATACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGA31930.1324213828392173No Hit
CTATTAGCCTTCCTGATTCATTAGCTGTTAGTCCATTCGATCTAAAAACT31840.13204813121204756No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC31470.13051365230034978No Hit
TCCTTGAAGAATCCCACCCAGGTATCTTTGAGAACTCATGCCTTGAAACA31230.12951831462789717No Hit
TCATATAGGAGACCGATTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC29920.12408542983242661No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT29640.12292420254789854No Hit
AAATAAAACAGGGACATTTGAATTCACAAGCTTTTTTTATCGATATGGAT29510.12238506130865338No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG29300.12151414084525736No Hit
GGCTAATAGATTTCCTCAAGGATGTGATGGAATCAATGGATAAAGAGGAA28970.12014555154563498No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG28800.11944052069431438No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT28730.11915021387318236No Hit
TTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCT28700.11902579666412579No Hit
ATATGTAATCATCGCCAAAAACATTCGAGGGTTTTGATTTTCATTCCACT28620.11869401743997493No Hit
GTTTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAATTT28530.11832076581280518No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG28420.11786456937959773No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT27830.11541769760148504No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT27790.11525180798940961No Hit
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC27440.11380027388374955No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC26810.11118751249356142No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT26130.10836738908827899No Hit
GATTACATATATCACAAAAAATCAACCTGAGTGGTTCAGAAACATCCTGA25740.10674996537054349No Hit
CTATTGTTCTTTGTGTGACCATTTTCTTGGTCATGTTGTCTCTTACTCTC25660.1064181861463926No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT25540.10592051731016629No Hit
AACTTATACAATATCCGGAACCTTCACATCCCTGAAGTCTGCTTAAAGTG25400.10533990366790227No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC25360.10517401405582684No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA24970.10355659033809132No Hit
CCATAGAAGTTTTTAGATCGAATGGACTAACAGCTAATGAATCAGGAAGG24820.10293450429280844No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG24810.10289303188978957No Hit
GGCTAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATCATAAG24590.10198063902337469No Hit
ATCCAGGAGTGTGTAGTTGCAACGGCATCATATTCCATACTTTTGGCTGG24300.10077793933582777No Hit
GCATTAGCCAACACCATAGAAGTTTTTAGATCGAATGGACTAACAGCTAA24230.10048763251469575No Hit
CATAGTGAATGCACCAAATCATGAGGGAATACAAGCAGGAGTGGATAGAT24220.10044616011167688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCCC1550.074.829185145
GGAAGCC1350.069.80593145
TGGACCC754.8639777E-967.65806145
ACTATTT851.3076715E-859.698284145
ACCGGCC1102.5465852E-1159.310635145
ACAGACC1154.0017767E-1156.73191145
GCCGACC1550.051.44507145
TCTCGGG3850.048.954807145
CGGTTCA450.00964562148.327183145
AGTACAA450.00964562148.327183145
ATTATCG450.00964641248.326183
ACATCTT1850.047.02104145
CGACGAA6950.044.850407145
TTATACT26900.042.582644
GTCGGCC700.001057599641.4233145
TATGTTT1252.723791E-740.594833145
AATGCCC907.491204E-540.27265145
CCTTATA29050.039.673242
TACTGGA29850.038.3735127
ACTGGAG29850.038.3735128
Produced by FastQC (version 0.11.5)