FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_CW_A7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_CW_A7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847799
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA29340.15878350404995348No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC28240.15283047560908952No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG25750.13935498395658835No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA22370.12106295111102452No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21930.11868173973467894No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG19800.10715451193555144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5200.098.9584661
CGAAAGC8900.057.8372234
AGCGAAA8900.057.8184282
TTACGTG3850.056.475292
TACGTGT3950.055.0559733
CGACGAA1750.053.852898145
GTTACGT4100.053.0316771
GCGAAAG10950.050.9749533
ACTCCGA5250.049.698261
GAGCAAA44700.049.2905921
AGTCGGC3700.045.0688975
TCGGCTG3950.042.216447
AACGGGT4850.038.860813
GGTGCGA3550.038.805018
GCCGACC1901.2914825E-1038.15489145
GTCGAAG1901.2914825E-1038.1528243
TCCGAGG6650.038.152823
GTAACGG4950.038.068531
ACGGGTT5000.037.7000924
GTCACGT3850.037.6501921
Produced by FastQC (version 0.11.5)