FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_795-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_795-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900750
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA28530.15009864527160333No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC26090.137261607260292No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG25900.1362620018413784No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG22260.11711166644745494No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20520.10795738524266736No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC19460.10238063922135998No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT19270.10138103380244641No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7800.073.418061
GTTACGT4150.061.1350671
CGAAAGC11900.060.3089183
GCGAAAG11950.059.451512
AGCGAAA12100.058.7098731
GAGCAAA51850.049.4908521
CAAAAGC68900.036.5150684
GATATAC7050.035.98923
ACGTGTT7450.034.0622754
GCCGACC2154.8748916E-1033.714798145
AAAGCAG91800.033.6447566
AAGCAGG90650.033.0309647
GTCGGCT2206.2391337E-1032.9554946
GCAAAAG77300.032.4481473
AATCGCT5000.031.8975542
AGTCGGC2301.0040822E-931.5234725
CGAAGCG5350.031.1699375
TAGTCGG2807.2759576E-1231.0715038
TCGGCTG2351.2641976E-930.8511377
GTTATGT12900.030.3441141
Produced by FastQC (version 0.11.5)