FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_549-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_549-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520851
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA13900.075.625641
GAGCAAA55200.075.5169751
GCAAAAG66900.061.7640843
GCGAAAG21250.061.394492
CAAAAGC67550.060.959144
AGCGAAA21400.060.3006631
CGAAAGC21850.060.050193
AGCAAAA70750.058.188582
AAAGCAG106850.048.0336956
AAAAGCA91450.045.3448685
AAGCAGG133650.036.393737
GACGAAA3150.032.21422145
GAAAGCA48900.028.3165514
GCAGGGG53700.028.0777329
TGGATCG2353.712274E-521.5946435
AGCAGGG125150.021.488998
GTAACGG2053.6689153E-421.218431
TAATACG1750.003660675120.7132284
CGAAGCG2104.3199165E-420.7111859
AGCAGGA49650.019.564048
Produced by FastQC (version 0.11.5)