FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_548-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_548-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017060
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA10890.10707332900713822No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC10530.10353371482508407No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG10390.10215719819872968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5150.076.016841
CGAAAGC5800.061.2449074
GCGAAAG6000.060.40573
GTTACGT1850.054.8630031
GAGCAAA27850.052.843811
AGCGAAA6700.051.928322
TACGTGT2250.048.3245663
TTACGCC604.9519504E-448.31981145
TTACGTG2150.047.1984142
ACGTGTT2550.039.8006174
CAAAAGC38200.038.5242844
GCGACTA951.0294065E-438.1547244
GCAAAAG39600.036.6095163
AAAGCAG50000.036.3919756
AAGCAGG53900.034.162287
AGCAAAA43300.033.3121762
TACTGCG1102.4373666E-432.9518055
CGAAGCG2700.032.221139
GTTATGT8350.032.124661
GGACCGT1153.1627654E-431.5206681
Produced by FastQC (version 0.11.5)