FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_545-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_545-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296221
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG12350.086.269572
AGCGAAA12500.084.6447141
CGAAAGC13950.076.909253
GAGCGAA4050.073.364481
GAGCAAA23150.039.1305581
GACGAAA3300.035.143646145
GCAAAAG29050.030.1946683
GAAAGCA38150.029.4585974
CAAAAGC30400.027.9053294
AAAGCAG63200.025.2394855
AGCAAAA34700.024.8556292
ACTCCGC1750.003658323320.7152448
TCGCCGG10400.019.51486145
AAGCAGG77500.019.4597176
AAAAGCA48700.018.610415
GTACTAG2801.4310541E-418.1174471
TGACGCC3002.4216993E-416.91288145
GTATTAT12700.016.548221
TCTCGGG10100.016.506207145
TACAGCG2700.002330565616.112485
Produced by FastQC (version 0.11.5)