FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_542-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_542-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356013
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAA64790.47779777922483047No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG61730.4552316238856117No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA58260.4296418987133604No Hit
ATATTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGG43040.31740108686273655No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA26870.19815444247215921No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC23110.17042609473507997No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT22840.1684349633816195No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT22620.16681256005657763No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC22540.16622259521110785No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA22010.1623140781098706No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT21230.15656192086654036No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20050.14785993939586123No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC19240.1418865453354798No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA17860.13170965175112628No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT17390.1282436082839914No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT17260.127284915410103No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT16470.12145901256108901No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG16320.12035282847583319No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG15540.11460067123250292No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT15110.1114296101881029No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC14240.10501374249361915No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC13980.1030963567458424No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC13800.10176893584353543No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG13570.10007278691280984No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG301.600824E-596.65257145
GAGCGAA8500.086.121791
GCAGGTA14750.069.789019
GAGCAAA49250.068.43171
GCGAAAG12900.061.8081322
AGCGAAA13000.061.3281561
CGAAAGC13150.060.6353073
GCAAAAG55850.059.9621853
GTTAAAC16900.058.326081
AGCAAAA59100.056.9079782
TTACGCC651.0947984E-555.761097145
ACTATAC18400.053.185186
AAGCAGG75950.052.1122177
GCAGGGT29250.051.5499429
AACTATA19250.050.836745
TTAAACT19800.050.1531262
CTATACA20050.048.8083467
CAAAAGC69350.048.7095534
ACCGCAT604.9492647E-448.3262868
TGACGCC1850.047.020172145
Produced by FastQC (version 0.11.5)