FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_540-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_540-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819785
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC27580.15155636517500695No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG23010.12644350843643617No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG22810.12534447750695824No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA22600.12419049503100642No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG19330.10622133933404221No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA18930.10402327747508634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6450.0107.9062961
AGCGAAA10250.067.894552
CGAAAGC11000.062.6132434
GTTACGT2650.060.1883051
GCGAAAG11950.059.4524273
GAGCAAA57450.056.9143561
TACGTGT2900.054.996633
TTACGTG2950.054.0615122
AAGCAGG86100.045.7201967
GATATAC6000.044.7055933
CAAAAGC75300.042.94134
GCAAAAG77400.041.773933
AAAGCAG93850.041.7129486
CTACTGA25150.040.643636
AGCAAAA79700.040.293312
GGTTATA4100.038.9021951
GTTATGT13150.036.3876081
TGACGCC1854.1945896E-935.263264145
CGATGTT2750.034.2734575
TATGTGT13400.034.0837333
Produced by FastQC (version 0.11.5)