FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_427-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_427-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726146
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT28250.1636593891826068No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT26930.1560122955995611No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA25020.14494718291500255No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA24900.14425199258927113No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC24870.14407819500783825No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT24140.1398491205263054No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG22780.1319702968346826No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT21520.1246707984145026No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT20430.11835615295577546No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20110.11650231208715832No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG20060.1162126494514369No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC18340.10624825478261979No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC18210.10549513192974407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA12850.098.1606751
CGAAAGC14700.085.807113
GCGAAAG15500.081.3783652
GAGCGAA4150.066.378421
TGACGCC2750.044.806877145
CAATTCG3950.038.539059
ACTACCG1001.3927466E-436.246115
GAAAGCA36150.035.8962174
TCTACAC7100.033.6935653
ATCCCGA3900.029.7395366
GTAGAAA23000.029.6272541
TAGCTCT18000.028.9977284
ATATGCG2252.8225259E-828.9968875
GAGCAAA30650.027.4358831
GTCATAC6400.027.1845821
TCTAGCT19600.026.9996532
TACCGTG1358.0915407E-426.8481927
CTCTAGC19750.026.794591
ACCTCGC1100.00967992326.3600438
AGTAGAA21050.026.1729621
Produced by FastQC (version 0.11.5)