FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_283-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_283-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480370
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG151420.6104734374307059No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA120050.4840003709124042No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA54500.2197252829214996No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT50240.20255042594451633No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT48640.1960997754367292No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT47780.1926325507887936No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT45770.184528921088386No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC45330.18275499219874455No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC44130.17791700431790416No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG41850.1687248273443075No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC41440.16707184815168705No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA40860.1647334873426142No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC39310.15848441966319543No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC37830.1525175679434923No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG36610.14759894693130463No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC34710.13993879945330737No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT32730.13195611944992078No Hit
GAATCTCCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC31970.1288920604587219No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT31770.1280857291452485No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG31450.12679559904369106No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG31380.12651338308397536No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG31190.12574736833617567No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT30830.12429597197192355No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC30180.12167539520313501No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT29660.1195789337881042No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA29410.11857101964626246No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA28250.11389429802811676No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC28050.11308796671464338No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT27530.11099150529961256No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT26720.10772586348004531No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC25510.10284755903353128No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC25350.10220249398275258No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA25240.1017590117603422No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA24880.1003076153960901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA14800.0122.93921
GCAGGTA35200.080.117819
AGCGAAA27700.071.443251
GAGCAAA105400.068.157151
CGAAAGC29650.067.9711763
GCGAAAG29750.067.497662
GACAGTA7950.067.481827
TCGCGCC451.1948976E-464.4369145
AAGCAGG147950.060.7615557
GCAAAAG117750.060.704463
GTAGAAA21250.059.6976851
GCAGGGT61700.057.9273839
AGCAGGT61150.053.2319158
AGCAAAA136000.052.7706222
CAAAAGC136800.052.3062134
AAAGCAG171650.052.2886926
TGACGCC1700.051.170475145
TTATAGG5950.049.9511261
CAATTCG4400.049.4300169
CAGTATG11200.048.5473379
Produced by FastQC (version 0.11.5)