FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_199-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_199-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065731
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC16450.1543541475287854No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA15460.14506474898449984No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA15160.14224977972865574No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG14740.138308822770474No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14570.13671367352549565No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG13160.12348331802302832No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA12400.1163520625748899No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT11440.10734416095618876No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA10830.1016203901359724No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC10810.10143272551891612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2400.0111.771531
CGAAAGC5200.066.9077763
AGCGAAA5350.065.047131
CAGTCGG2200.062.6081434
GCGAAAG5750.060.505062
GTTACGT1450.060.000371
GTAATGC1950.059.487551
AGTCGGC2100.058.6880845
TTACGTG1500.057.9840162
GTCACGT2200.056.023071
GGACCGT651.0936528E-555.7695771
CGAAGCG1301.8189894E-1255.7669565
GATATAC3950.055.0507053
GTCGGTA400.006050844754.375341
GTCGTCT400.00605984454.354916145
TACGTGT1650.052.7152183
GGTTATA1700.051.176791
GAGCAAA29700.046.8689731
AAGCGGG2900.044.996057
AAGCAGG36200.044.2571647