FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_197-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_197-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745825
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG39610.22688413787177983No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA27560.15786232869846634No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC22720.13013904600976614No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA20860.11948505720791029No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC17510.10029642146263229No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGT100.0070945676144.977741
GAGCGAA4150.0113.536791
AGCGAAA8150.066.707541
CGAAAGC9100.059.743573
GCAGGTA11400.056.598679
GTTACGT2450.056.215861
ACTCCGA1700.055.432661
GCGAAAG10000.054.368212
TCGCGCC400.006055315554.366653145
TACGTGT2550.054.0113143
TTACGTG2600.052.974152
TACGTTA450.00964508948.3272972
AAGCAGG59600.048.1692627
GAGCAAA50250.048.03741
TCCGAGG2050.045.968553
AGCAGGT20750.040.528548
TGAAGCG1502.600973E-838.6673775
ACGATCC6200.038.582783145
GGTCGTT1553.474088E-837.4178967
CAAGGTA4950.035.152164
Produced by FastQC (version 0.11.5)