FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_156-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_156-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282381
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8100.093.079931
GAGCAAA40450.075.810531
GCGAAAG16500.068.971962
AGCGAAA16500.068.540671
CGAAAGC17850.064.572813
CAAAAGC48400.062.461934
GCAAAAG51100.059.015113
AGCAAAA53350.056.3859062
CGAAGCG553.219832E-452.7256559
AAAAGCA72200.042.474465
AAAGCAG89150.042.2056586
AAGCAGG108250.033.6202167
GACGAAA3900.029.735712145
GAAAGCA40800.029.3189534
GACCGTA1255.1555654E-428.999119
TGACGCC2104.999856E-727.611734145
GCAGGGG40350.026.5915019
AGCAGGG89700.022.8727658
CGAATAA2205.915151E-419.76749145
GTATTAG6800.019.1898381
Produced by FastQC (version 0.11.5)