FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n02_TR_123-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n02_TR_123-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292577
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC16520.12780669933009794No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC15620.1208438646208311No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG14830.11473204304269687No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA14590.11287528712022572No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT13320.10304995369714919No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA13260.1025857647165314No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC13110.10142529226498692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3650.0115.1827551
TGACGCC1450.074.98521145
AGCGAAA6200.065.4684142
GCGAAAG7150.058.801863
CGAAAGC7000.057.997544
GGTGCGA2650.051.9769138
TTATTCG450.00964138648.331284
GGTCGTT1551.4551915E-1146.7703977
ACTCCGA3900.044.6065831
CGAAGCG3150.043.7283025
AGTCCGC1002.7384795E-643.491417145
GAGCAAA33500.042.6259161
TTACGTG2300.040.968592
GTTATGT7000.040.384891
AGGTCGT1807.2759576E-1140.2745066
GTATATC4200.039.6945461
GTTACGT2400.039.2630841
AAGCAGG47350.038.7349747
TCCGAGG4550.038.235693
TACATCG951.02931444E-438.1562774