FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11135825
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405530.36416700154680953No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG204920.18401869641450005No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA156760.14077089034714535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA22350.0101.9111561
GAGCAAA171350.064.818021
AGCAAAA193400.057.347782
GCAAAAG194900.056.7902763
CGAAAGC44650.056.670523
GCAGGTA61350.054.4033789
GCAGGGT107100.052.884099
AGCAGGG172900.042.5289768
AAAGCAG375500.036.1377456
TCGTACT3750.034.704752
GGTAGTT17150.034.116987
GTCATAC27150.033.823811
CAAAAGC336800.033.571854
ACAAGGG67100.032.9692843
AAGCAGG428900.031.3766447
GTAGTTT19050.030.7129968
TCTAGCT33100.030.362292
GTTACGT1204.1241074E-430.1284961
TAGCTCT32900.030.1047694
GAAAGCA85700.029.8629744
Produced by FastQC (version 0.11.5)