FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_PLASMID_CONTROL_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_PLASMID_CONTROL_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411242
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA67940.2817635061101291No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG61210.25385257887843693No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG60890.25252546198183345No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT59930.24854411129202292No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG59510.24680227036523086No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG56720.23523146992296914No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC56160.23290901535391303No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT50010.20740348749731466No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT47220.19583268705505294No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA46570.1931369808588271No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA42350.17563562678486855No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG40700.16879268028675679No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT38560.15991758604072093No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG37970.15747071426260822No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAACCTTTAGGAA37670.15622654217204246No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT37370.15498237008147667No Hit
GAATAAGAGAGGCTATCTAATAAGAGCTTTGACATTGAACACGATGACCA36900.1530331671395903No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAATTTGCAGGTCCT36750.15241108109430743No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT36390.15091807458562848No Hit
GTATAAGTTTGGTCCCCCATCTGATACCAATAGTCCTGCCCTTGATTGGG36290.1505033505554399No Hit
ATGTAATCATCGCCAAAAACATTCGAGGGTTTTGATTTTCATTCCACTTA35630.14776617195619518No Hit
GATATACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGA34950.14494604855091278No Hit
TCCTTGAAGAATCCCACCCAGGTATCTTTGAGAACTCATGCCTTGAAACA33730.13988641538261196No Hit
CTCTTATTCACTCTTTGTTTTTTCTTCCCTATTGTTCTTTGTGTGACCAT33670.1396375809644988No Hit
TCCCTGAAGTCTGCTTAAAGTGGGAGCTAATGGATGAGAATTATCAGGGA33110.1373151263954427No Hit
CTATTAGCCTTCCTGATTCATTAGCTGTTAGTCCATTCGATCTAAAAACT33050.13706629197732953No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC32170.13341672051166992No Hit
TCATATAGGAGACCGATTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC32140.13329230330261335No Hit
AAATAAAACAGGGACATTTGAATTCACAAGCTTTTTTTATCGATATGGAT31260.12964273183695374No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG30280.12557843634110555No Hit
GGCTAATAGATTTCCTCAAGGATGTGATGGAATCAATGGATAAAGAGGAA30280.12557843634110555No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT29810.12362923339921915No Hit
ATATGTAATCATCGCCAAAAACATTCGAGGGTTTTGATTTTCATTCCACT29490.12230211650261567No Hit
GTTTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAATTT29400.12192886487544592No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT29380.12184592006940821No Hit
TTCTTAATCCGTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCT28990.1202284963516727No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT28540.11836223821582405No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG28490.11815487620072974No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG28380.11769867976752231No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC27570.1143394151229947No Hit
AACTTATACAATATCCGGAACCTTCACATCCCTGAAGTCTGCTTAAAGTG27140.11255610179318376No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC27050.11218285016601404No Hit
GATTACATATATCACAAAAAATCAACCTGAGTGGTTCAGAAACATCCTGA26560.11015070241808993No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT26080.1081600270731847No Hit
AATAAGAGCTTTGACATTGAACACGATGACCAAAGATGCAGAGAGAGGTA25970.10770383063997722No Hit
GCATTAGCCAACACCATAGAAGTTTTTAGATCGAATGGACTAACAGCTAA25580.10608640692224174No Hit
GGGTACACCATGGACACAGTCAACAGAACACACCAATATTCAGAGAAGGG25570.10604493451922287No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT25560.10600346211620401No Hit
ATCCAGGAGTGTGTAGTTGCAACGGCATCATATTCCATACTTTTGGCTGG25550.10596198971318516No Hit
CTATTGTTCTTTGTGTGACCATTTTCTTGGTCATGTTGTCTCTTACTCTC25410.10538137607092113No Hit
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC25400.10533990366790227No Hit
CCATAGAAGTTTTTAGATCGAATGGACTAACAGCTAATGAATCAGGAAGG25300.10492517963771367No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG25270.10480076242865709No Hit
ATTCCAGACACTCCAAAACTGGGAAGCTCCATGCTAAAATTAGCCACAAA25070.10397131436827992No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA24940.10343217312903474No Hit
TTCACTATGAGGGCAAAATCGTCGGAGGATTGGAGCCCATCCCACCAGTA24880.1031833387109216No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT24770.10272714227771414No Hit
CATAGTGAATGCACCAAATCATGAGGGAATACAAGCAGGAGTGGATAGAT24380.10110971855997863No Hit
TTATTGGTGCGATGCTCAGGATGTTTCTGAACCACTCAGGTTGATTTTTT24360.10102677375394091No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAGG100.007096734144.964072
AGGCCCC651.6370905E-1189.162415145
ATAATTA3800.053.3801278
TAATTAA3900.052.011419
AATAATT4100.051.2412037
AGAGATA700.001060930841.39683145
TTATACT25400.039.379194
TATATAT25100.038.4068152
GTATATA25200.038.25521
TATACTG49700.037.1881985
CCTTATA27050.036.9778942
TACTGAT23200.036.8467527
ACTGATG23250.036.455928
CATATAC23900.035.7853663
CTGATGA23800.035.6134539
TGAGTAA27200.035.4232068
TACTGGA28350.035.263977
ACTGGAG28350.035.263978
GTACGGA1707.846211E-834.1098981
GAGTAAA28450.033.866839
Produced by FastQC (version 0.11.5)