FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_CW_A7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_CW_A7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847799
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA31350.16966131056462308No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC28420.15380460753577635No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG26180.14168207689256243No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA24890.13470079808464017No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC22630.12247003056068329No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21870.11835702909245No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG18930.10244620762323174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5100.0108.0597151
AGTCGGC4700.066.336985
TATTCGC451.1930046E-464.456671
TCGGCTG5150.060.5012367
AGCGAAA9450.057.547482
GTTACGT3700.056.8351061
GTCGGCT5500.056.687976
CGAAAGC9650.056.3532644
TACGTGT3850.054.6163143
CAGTCGG5850.054.535834
GTCGAAG2150.053.9593433
GAAGGCG2800.051.7913324
GCGAAAG11800.051.615773
TTACGTG4100.051.2874372
GCGATTA1750.049.68739145
CGGCTGG6600.049.4050758
GAGCAAA47700.047.886441
CAGCAAC8800.046.934827
TCGAAGG3150.046.036744
AGTCCGC2750.044.793934145
Produced by FastQC (version 0.11.5)