FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_795-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_795-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900750
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG28930.15220307773247405No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA28250.14862554254899382No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC27270.1434696830198606No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC24420.1284756017361568No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT24200.1273181638826779No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG22930.12063659081941339No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG22330.11747994212810732No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT19080.10038142838353281No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7700.072.4676061
GTTACGT5150.060.5069271
TTACGTG5050.060.270082
AGCGAAA11550.059.6053851
TACGTGT5350.058.244993
GCGAAAG12600.056.938832
CGAAAGC12600.056.363693
ACGTGTT6050.051.50594
GAGCAAA53750.049.884671
GGTGCGA2900.042.4586568
CAAAAGC68300.038.9393964
AGACGAC4350.038.31623
GTAACGG4200.037.959221
GTGCGAT3250.037.886199
GTCGTCT1803.2341632E-936.214737145
GTTATGT12850.036.092811
CGTGTTG8650.036.0243575
CGACTCC4650.035.844196
CGGGTTG4450.035.826685
CGAAGCG3250.035.6763575
Produced by FastQC (version 0.11.5)