FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_549-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_549-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520851
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA46600.079.1911541
GAGCGAA13950.069.122941
GCAAAAG59250.061.1723253
CAAAAGC60750.059.303934
GCGAAAG19100.058.8301932
AGCGAAA19450.057.7772561
AGCAAAA63000.057.4198382
CGAAAGC20350.055.5691223
AAAGCAG100300.045.1702466
AAAAGCA85400.042.525875
AAGCAGG122700.034.9537247
GAAAGCA44700.027.892944
GTAACGG2756.7702786E-926.3640481
CGGGTTG3151.036824E-925.3136925
GCAGGGG50650.024.0295759
TAACGGG3102.4221663E-823.3851552
GCGAAGC1800.00431179920.1358913
ACGGGTT3651.3634417E-719.8600584
AGCAGGG120250.019.7005778
CGACGTT500.001398704117.39226235-39
Produced by FastQC (version 0.11.5)