FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_548-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_548-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017060
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC11790.11592236446227361No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11250.11061294318919239No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA11050.10864649086582895No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC10200.10028906849153443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4850.082.208661
ACGCGTG350.003568562762.1338775
GCGAAAG7500.054.1255073
CGAAAGC7750.051.4441764
GAGCAAA24200.050.0262221
AGCGAAA8250.049.205012
GGCCGTA1203.6252459E-948.326352
GTAACGG1801.8189894E-1244.3013341
AACGGGT1953.6379788E-1240.8915253
CGAAGCG2550.039.772739
GTCGTCT1105.2928062E-639.514473145
GTTACGT1901.2914825E-1038.1542551
GCGACTA1354.977119E-737.587164
CAAAAGC34150.034.811954
TAACGGG2302.7284841E-1134.6689032
GCAAAAG35600.033.597673
GTTATGT7700.032.95141
AAAGCAG49150.032.8894466
TAGTCGG1601.8886767E-631.69398
AGCAAAA37950.031.326172
Produced by FastQC (version 0.11.5)