FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_545-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_545-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296221
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9600.079.311211
GCGAAAG11050.068.9038542
GAGCGAA4050.068.036991
CGAAAGC11600.065.6368563
GAGCAAA24650.044.125621
TACGAAC1209.594714E-636.2341779
GCAAAAG30650.035.2510723
CAAAAGC30200.035.0560044
TATACGA1301.6601112E-533.446937
AGCAAAA35050.030.6189422
AAAAGCA46600.023.4967355
TATAGGC4154.3655746E-1122.7149473
AAAGCAG60600.022.3761546
GTATAGG5201.8189894E-1220.9172421
GAAAGCA38600.020.8522134
TATAGCG1850.00504239419.5981372
ATATACG2659.339428E-519.1544066
TATCCCG1900.00588266119.0823965
TATACTG10000.018.1282755
GTACAGG6900.017.8655471
Produced by FastQC (version 0.11.5)