FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_540-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_540-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819785
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC28570.1569965682759227No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG24780.13616993216231588No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA24310.13358720947804273No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23690.13018021359666113No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA21740.1194646620342513No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG20340.11177144552790576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7200.0116.793161
AGCGAAA11700.071.24922
CGAAAGC11900.070.051734
GTTACGT3150.066.7389451
TTACGTG3150.066.735272
GCGAAAG13000.065.797083
TACGTGT3400.061.828273
GAGCAAA53900.055.4115561
GATATAC6700.047.604273
AAGCAGG86400.044.60587
CGAAGCG2650.043.7665335
TATACTG9250.039.966535
AAAGCAG95700.039.766326
GTACGGA1303.7036807E-739.0343131
TAGTCGG2600.039.0075048
CAAAAGC77900.038.058694
GCAAAAG78450.037.6070673
AACGGGT5900.036.8584633
CTACTGA26550.036.585446
AGCAAAA80950.036.3560942
Produced by FastQC (version 0.11.5)