FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_427-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_427-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726146
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT32200.1865427374045996No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT29290.16968437200561248No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA28440.16476010719834824No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA27420.1588509894296311No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC26010.15068250310228684No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT25680.1487707297065254No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT22900.13266548716041401No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC21420.12409147314305974No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20140.11667610966859118No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC20070.11627058197858119No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG19840.11493813385426262No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT18880.1093766112484112No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG17650.10225091040966408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA13900.098.048581
CGAAAGC15950.085.4442443
GCGAAAG16400.083.102152
GAGCGAA3700.080.330481
CCCTACG450.00964379948.328912
ACTACCG450.00964490348.327515
CAATTCG2850.043.2166069
GAAAGCA37300.037.3145374
TCTACAC7600.037.1994633
GGTCGTT1957.044946E-933.439097
AGGTCGT2008.969437E-932.621076
GTAGAAA22500.029.6417311
GTCATAC8550.027.9798951
CTACACT10800.027.519834
AGTAGAA22150.027.1645551
GAGCAAA30000.026.8225441
CAGGGTG14050.025.7834039
ACAAGGG23350.025.767773
TAGAAAC25900.025.470642
GTATACA5150.025.3374881
Produced by FastQC (version 0.11.5)