FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_199-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_199-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065731
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC17100.1604532475831143No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA16160.1516330105814694No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15830.1485365444000409No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA15350.14403259359069032No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG15220.14281277357982455No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG13040.12235733032069068No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA12940.12141900723540931No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT12560.11785337951134009No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC11930.11194194407406746No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA11330.10631200556237926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3200.0113.254841
AGCGAAA5150.071.776012
CGAAAGC5100.071.0585254
ATACCGG350.003578501862.0904778
GCGAAAG6000.061.6077463
TTACGTG2600.061.3289762
GTTACGT2650.060.174651
TACGTGT2700.059.057533
GATATAC2800.054.359773
GATACCG400.006071214654.3291637
TACCGGA400.006071214654.3291639
TAATCGT1405.456968E-1251.7420659
GCGACTA1550.051.4372024
GGTTATA1501.0913936E-1148.322071
GATACGA450.00964818948.3220671
GGGTTAC1800.048.3220671
AAGCAGG35550.047.2737587
AGCAGGT8500.046.872228
GAGCAAA29800.045.484361
AGACGAC1950.044.602893