Basic Statistics
Measure | Value |
---|---|
Filename | HLCT5BCX2_l01_n01_TR_199-7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1065731 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC | 1710 | 0.1604532475831143 | No Hit |
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA | 1616 | 0.1516330105814694 | No Hit |
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG | 1583 | 0.1485365444000409 | No Hit |
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA | 1535 | 0.14403259359069032 | No Hit |
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG | 1522 | 0.14281277357982455 | No Hit |
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG | 1304 | 0.12235733032069068 | No Hit |
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA | 1294 | 0.12141900723540931 | No Hit |
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT | 1256 | 0.11785337951134009 | No Hit |
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC | 1193 | 0.11194194407406746 | No Hit |
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA | 1133 | 0.10631200556237926 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GAGCGAA | 320 | 0.0 | 113.25484 | 1 |
AGCGAAA | 515 | 0.0 | 71.77601 | 2 |
CGAAAGC | 510 | 0.0 | 71.058525 | 4 |
ATACCGG | 35 | 0.0035785018 | 62.090477 | 8 |
GCGAAAG | 600 | 0.0 | 61.607746 | 3 |
TTACGTG | 260 | 0.0 | 61.328976 | 2 |
GTTACGT | 265 | 0.0 | 60.17465 | 1 |
TACGTGT | 270 | 0.0 | 59.05753 | 3 |
GATATAC | 280 | 0.0 | 54.35977 | 3 |
GATACCG | 40 | 0.0060712146 | 54.329163 | 7 |
TACCGGA | 40 | 0.0060712146 | 54.329163 | 9 |
TAATCGT | 140 | 5.456968E-12 | 51.742065 | 9 |
GCGACTA | 155 | 0.0 | 51.437202 | 4 |
GGTTATA | 150 | 1.0913936E-11 | 48.32207 | 1 |
GATACGA | 45 | 0.009648189 | 48.322067 | 1 |
GGGTTAC | 180 | 0.0 | 48.322067 | 1 |
AAGCAGG | 3555 | 0.0 | 47.273758 | 7 |
AGCAGGT | 850 | 0.0 | 46.87222 | 8 |
GAGCAAA | 2980 | 0.0 | 45.48436 | 1 |
AGACGAC | 195 | 0.0 | 44.60289 | 3 |