FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_197-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_197-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745825
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG39440.22591038620709408No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA28070.1607835836925236No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC22590.12939441238382998No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA21720.12441109504102645No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC18780.1075709191929317No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC17820.10207208626294159No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3950.0130.326071
TTACGTG1950.074.362332
AGCGAAA7900.069.7519761
TACGTGT2100.069.0507353
GTTACGT2200.065.913951
CGAAAGC9150.061.0136833
GCGAAAG10150.055.002482
GAGCAAA50350.048.6729051
AACGGGT2700.045.6502083
AAGCAGG63800.045.425357
AGTCGGC2350.043.1934365
GCAGGTA12350.042.826729
ACGGGTT2950.041.7815444
CAGTCGG2700.040.2795944
GACAGTA2350.040.080627
GCGATTA1451.940316E-839.974304145
CTACTGA18100.038.8553436
CTACACT7700.038.6056334
TCTACAC8300.038.4354673
GTATATC2950.036.8671261
Produced by FastQC (version 0.11.5)