FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_156-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_156-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282381
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9250.095.601711
GAGCAAA40250.073.295291
AGCGAAA17750.069.422161
GCGAAAG18450.067.181132
CGAAAGC19250.064.76323
CAAAAGC47650.061.1496244
AGCAAAA50800.057.0747262
GCAAAAG50800.057.0725023
GCGACTA752.5547215E-548.321384
AAAAGCA70300.041.9633065
AAAGCAG91850.041.587436
AAGCAGG108200.033.272757
GAAAGCA44250.029.320434
GCAGGGG43300.026.2646339
TACGACC1400.001001056925.8864544
AGCAGGG96050.021.945998
GAGCGAC1700.003097002921.319092
GTATAGA11000.019.768611
AGCAGGT21450.019.5866558
TTCGCTC4202.8667273E-818.9833984
Produced by FastQC (version 0.11.5)