FastQCFastQC Report
Thu 18 Oct 2018
HLCT5BCX2_l01_n01_TR_123-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLCT5BCX2_l01_n01_TR_123-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292577
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC17450.13500162852967368No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG17000.13152021117504026No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA16750.12958609042246613No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC16250.12571784891731788No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC14530.11241109813960792No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA13740.1062992765614737No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12960.10026481981344246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3800.0110.637871
TTACGTG2900.062.486272
TACGTGT2900.062.486273
CGAAAGC6950.060.4903034
GTTACGT3050.059.4154741
AGCGAAA7100.059.212342
GTCGAAG1850.054.8528183
GGTTATA2000.054.365161
GCACGTA400.006056546254.3630526
GCGAAAG7750.054.2461433
AGTCCGC1457.2759576E-1249.963875145
ACTCCGA5200.048.7892461
TCCGAGG5100.048.3227163
GAGCAAA30950.045.6702461
AGACGAC2400.045.3025473
GGTCGTT1151.4210673E-744.0985537
GTAACTC2050.042.4297033
AACGGGT3550.040.8360983
CCCGCTC4550.039.8264123
ACGTGTT4600.039.393524