FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_5n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_5n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19933831
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT50200.09.2764161
GGGAAAT71550.09.1998441
GGAAAAT114100.09.1752471
CGGGGAT68500.09.09833051
CGGGAAT33550.08.9750771
CGGAAAT32300.08.6720091
CGGTAAT22250.08.6549571
CCGGGAT74600.08.2604951
CGGCAAT61900.08.2583721
CTCGGAT45400.08.09777451
GGCGAAT34600.07.9943521
CGCGAAT22400.07.97176361
CCGGAAT37800.07.96597431
GGGGAAT52600.07.9212461
GGGAGAT95350.07.8582411
GCCGAAT34600.07.58957431
GGAAGAT186650.07.3159191
CCCGAAT25000.07.28275351
CGGAGAT142700.07.18912271
CGGGTAT19550.07.1638331
Produced by FastQC (version 0.11.2)