FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_5n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_5n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6210448
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65310.10516149559580887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT20700.010.8236731
CGGAAAT11250.09.3354191
CGACAAT18050.09.3095581
CTCGAAT12450.09.27918151
GCCAAAT24350.09.0574651
CGGGAAT10450.09.0450831
GGGGAAT17600.08.9508631
CCCGGAT13400.08.8825811
GGAAAAT34000.08.85491851
CGGACAT16250.08.617311
CCCGACT9202.2919266E-108.3714351
GGGACAT16650.08.200031
CTCGGAT14300.08.0787281
CTTAAAT17600.07.95632271
GTCGAAT12051.8189894E-127.84407571
CGACGAT45100.07.83988861
AAATCTA20000.07.6970694
CGCGAAT6007.2026276E-57.5850271
CCGGAAT13850.07.58292151
CGGATAT9756.2464096E-97.54014541
Produced by FastQC (version 0.11.2)