FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_5n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_5n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25715247
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290920.11313132632947294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT27450.09.3109691
GCCGAAT45550.09.2237431
GCGGAAT33400.08.595711
GGGAAAT96200.08.4799871
CGGGGAT88150.08.4600251
CCGGAAT59800.08.1967411
GGGGTAT35200.08.1561561
GGGGAAT73100.08.1422781
CTCGGAT60100.07.9810581
GGAAAAT141100.07.8658671
CGCGAAT27650.07.8507461
GGGACAT68350.07.68364141
GGGAGAT130600.07.66720771
CCGGGAT88400.07.6439771
GGGATAT68200.07.5978671
CGGAAAC50950.07.55897431
CGGCAAT84350.07.51290371
CGGGCAT61900.07.35304261
CGGGACT29350.07.2767271
GGAAAAC104200.07.2577231
Produced by FastQC (version 0.11.2)