FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_5k3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_5k3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26589801
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312830.11765037278767149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT30300.010.8610391
CGGGCAT64150.09.4959581
CGGGACT33350.09.3428711
CGGGAAT51250.09.2220211
GCCGAAT48350.08.9062481
CGGGGAT92700.08.6862891
CCGGGAT98600.08.5570941
CGGACAT73050.08.48281
GCGGAAT34800.08.3499721
CGGGTAT25200.08.33561
GGAAAAT150900.08.2593711
CCGGAAT60100.08.0970441
GGGAAAT97300.08.0957271
CGGAAAT48000.08.0230151
CCCGGAT65200.07.89325051
CTCGGAT63800.07.7372141
CGGAGAT195350.07.70621731
GCCGGAT94550.07.5536141
GGGATAT73100.07.47125151
GGGAGAT141000.07.39917561
Produced by FastQC (version 0.11.2)